Identification of lignin and polysaccharide modifications in Populus wood by chemometric analysis of 2D NMR spectra from dissolved cell walls.
Identifieur interne : 003595 ( Main/Exploration ); précédent : 003594; suivant : 003596Identification of lignin and polysaccharide modifications in Populus wood by chemometric analysis of 2D NMR spectra from dissolved cell walls.
Auteurs : Mattias Hedenström [Suède] ; Susanne Wiklund-Lindström ; Tommy Oman ; Fachuang Lu ; Lorenz Gerber ; Paul Schatz ; Björn Sundberg ; John RalphSource :
- Molecular plant [ 1674-2052 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Bois, Lignine, Paroi cellulaire, Polyosides, Populus.
- métabolisme : Paroi cellulaire.
- Analyse multifactorielle, Résonance magnétique nucléaire biomoléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Lignin, Polysaccharides.
- chemistry : Cell Wall, Populus, Wood.
- metabolism : Cell Wall.
- Multivariate Analysis, Nuclear Magnetic Resonance, Biomolecular.
Abstract
2D 13C-(1)H HSQC NMR spectroscopy of acetylated cell walls in solution gives a detailed fingerprint that can be used to assess the chemical composition of the complete wall without extensive degradation. We demonstrate how multivariate analysis of such spectra can be used to visualize cell wall changes between sample types as high-resolution 2D NMR loading spectra. Changes in composition and structure for both lignin and polysaccharides can subsequently be interpreted on a molecular level. The multivariate approach alleviates problems associated with peak picking of overlapping peaks, and it allows the deduction of the relative importance of each peak for sample discrimination. As a first proof of concept, we compare Populus tension wood to normal wood. All well established differences in cellulose, hemicellulose, and lignin compositions between these wood types were readily detected, confirming the reliability of the multivariate approach. In a second example, wood from transgenic Populus modified in their degree of pectin methylesterification was compared to that of wild-type trees. We show that differences in both lignin and polysaccharide composition that are difficult to detect with traditional spectral analysis and that could not be a priori predicted were revealed by the multivariate approach. 2D NMR of dissolved cell wall samples combined with multivariate analysis constitutes a novel approach in cell wall analysis and provides a new tool that will benefit cell wall research.
DOI: 10.1093/mp/ssp047
PubMed: 19825670
Affiliations:
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first="Tommy" last="Oman">Tommy Oman</name></author><author><name sortKey="Lu, Fachuang" sort="Lu, Fachuang" uniqKey="Lu F" first="Fachuang" last="Lu">Fachuang Lu</name></author><author><name sortKey="Gerber, Lorenz" sort="Gerber, Lorenz" uniqKey="Gerber L" first="Lorenz" last="Gerber">Lorenz Gerber</name></author><author><name sortKey="Schatz, Paul" sort="Schatz, Paul" uniqKey="Schatz P" first="Paul" last="Schatz">Paul Schatz</name></author><author><name sortKey="Sundberg, Bjorn" sort="Sundberg, Bjorn" uniqKey="Sundberg B" first="Björn" last="Sundberg">Björn Sundberg</name></author><author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19825670</idno><idno type="pmid">19825670</idno><idno type="doi">10.1093/mp/ssp047</idno><idno type="wicri:Area/Main/Corpus">003420</idno><idno 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last="Ralph">John Ralph</name></author></analytic><series><title level="j">Molecular plant</title><idno type="ISSN">1674-2052</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (chemistry)</term><term>Cell Wall (metabolism)</term><term>Lignin (chemistry)</term><term>Multivariate Analysis (MeSH)</term><term>Nuclear Magnetic Resonance, Biomolecular (MeSH)</term><term>Polysaccharides (chemistry)</term><term>Populus (chemistry)</term><term>Wood (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse multifactorielle (MeSH)</term><term>Bois (composition chimique)</term><term>Lignine (composition chimique)</term><term>Paroi cellulaire (composition chimique)</term><term>Paroi cellulaire (métabolisme)</term><term>Polyosides (composition chimique)</term><term>Populus (composition chimique)</term><term>Résonance magnétique nucléaire biomoléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Bois</term><term>Lignine</term><term>Paroi cellulaire</term><term>Polyosides</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Multivariate Analysis</term><term>Nuclear Magnetic Resonance, Biomolecular</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse multifactorielle</term><term>Résonance magnétique nucléaire 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We demonstrate how multivariate analysis of such spectra can be used to visualize cell wall changes between sample types as high-resolution 2D NMR loading spectra. Changes in composition and structure for both lignin and polysaccharides can subsequently be interpreted on a molecular level. The multivariate approach alleviates problems associated with peak picking of overlapping peaks, and it allows the deduction of the relative importance of each peak for sample discrimination. As a first proof of concept, we compare Populus tension wood to normal wood. All well established differences in cellulose, hemicellulose, and lignin compositions between these wood types were readily detected, confirming the reliability of the multivariate approach. In a second example, wood from transgenic Populus modified in their degree of pectin methylesterification was compared to that of wild-type trees. We show that differences in both lignin and polysaccharide composition that are difficult to detect with traditional spectral analysis and that could not be a priori predicted were revealed by the multivariate approach. 2D NMR of dissolved cell wall samples combined with multivariate analysis constitutes a novel approach in cell wall analysis and provides a new tool that will benefit cell wall research.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19825670</PMID><DateCompleted><Year>2010</Year><Month>02</Month><Day>04</Day></DateCompleted><DateRevised><Year>2009</Year><Month>10</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1674-2052</ISSN><JournalIssue CitedMedium="Print"><Volume>2</Volume><Issue>5</Issue><PubDate><Year>2009</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Molecular plant</Title><ISOAbbreviation>Mol Plant</ISOAbbreviation></Journal><ArticleTitle>Identification of lignin and polysaccharide modifications in Populus wood by chemometric analysis of 2D NMR spectra from dissolved cell walls.</ArticleTitle><Pagination><MedlinePgn>933-42</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/mp/ssp047</ELocationID><Abstract><AbstractText>2D 13C-(1)H HSQC NMR spectroscopy of acetylated cell walls in solution gives a detailed fingerprint that can be used to assess the chemical composition of the complete wall without extensive degradation. We demonstrate how multivariate analysis of such spectra can be used to visualize cell wall changes between sample types as high-resolution 2D NMR loading spectra. Changes in composition and structure for both lignin and polysaccharides can subsequently be interpreted on a molecular level. The multivariate approach alleviates problems associated with peak picking of overlapping peaks, and it allows the deduction of the relative importance of each peak for sample discrimination. As a first proof of concept, we compare Populus tension wood to normal wood. All well established differences in cellulose, hemicellulose, and lignin compositions between these wood types were readily detected, confirming the reliability of the multivariate approach. In a second example, wood from transgenic Populus modified in their degree of pectin methylesterification was compared to that of wild-type trees. 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Plant</MedlineTA><NlmUniqueID>101465514</NlmUniqueID><ISSNLinking>1674-2052</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015999" MajorTopicYN="N">Multivariate Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019906" 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last="Schatz">Paul Schatz</name><name sortKey="Sundberg, Bjorn" sort="Sundberg, Bjorn" uniqKey="Sundberg B" first="Björn" last="Sundberg">Björn Sundberg</name><name sortKey="Wiklund Lindstrom, Susanne" sort="Wiklund Lindstrom, Susanne" uniqKey="Wiklund Lindstrom S" first="Susanne" last="Wiklund-Lindström">Susanne Wiklund-Lindström</name></noCountry><country name="Suède"><noRegion><name sortKey="Hedenstrom, Mattias" sort="Hedenstrom, Mattias" uniqKey="Hedenstrom M" first="Mattias" last="Hedenström">Mattias Hedenström</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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